Reaching your goals in obesity studies can be a challenge or a success depending on the reliability of your research models. The Lepob mutation was discovered in 1949 in an outbred mouse stock and was subsequently transferred to a C57BL/6 background. This model has since been well characterized as a model of obesity, exhibiting commonly published metabolic symptoms including hyperinsulinemia and hyperphagia.
Mutation backcrossed onto the C57BL/6J strain at The Jackson Laboratories, Bar Harbor, Maine, in the 1950’s. Acquired by the Laboratory Animals Centre, Carshalton, United Kingdom, in 1974. To Olac, United Kingdom, in 1978. To Harlan Laboratories, in 2001. Harlan became Envigo in 2015.
|Over 8 weeks, add per week||4.00||USD|
|* Genetic testing (at additional cost) is required to determine Lepob / Lep+ from Lep+ / Lep+|
|Over 8 weeks, add per week||5.65||USD|
Envigo uses well-established techniques to successfully produce time mated rats, mice, hamsters, and rabbits. We use an Impedence Meter for determining the stage of estrus in rats prior to breeding. Time mated rats and mice are determined by observation of a vaginal plug. Plug date is considered to be day zero (0) of gestation.
Due to the natural variation in the length of gestation, the exact day of parturition cannot be guaranteed. In addition, Envigo cannot guarantee the minimum number of offspring per litter.
Please be aware that late-term pregnant animals may deliver their litter prematurely while in transit, and depending on the circumstances involved, requests for credit or replacement of these animals may be declined at our sole discretion.
If problems regarding gestational age or pregnancy are encountered, customers should immediately contact Envigo’s Customer Service Department and provide detailed information regarding the animals involved.
|Expected Pregnancy Rate|
|Stock or Strain||Timed mated <13 days gestation (at shipping)||Timed mated ≥ 13 days gestation and over (at shipping)||Untimed pregnant ≥ 13 days gestation (at shipping)|
* Plug guarantee only; no guaranteed pregnancy. Plug date = Day 0.
** Untimed pregnant rodents will be selected from our breeding colonies on the basis of palpation or visual confirmation. A variation of three to four days gestation can be expected. Therefore Envigo cannot be held responsible for actual gestation and/or exact day of littering.
To avoid charges, cancellations for timed mated animals must be received one week prior to mating date.