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Cryopreservation (embryo and sperm)

You want to preserve the integrity of your research colony’s genetic lineage, but you don’t have the capacity, time, resources or desire to maintain it until some undefined future date. That’s understandable, particularly as the number of genetically altered lines developed and maintained for research in animal facilities continually increases. Laboratories often develop their own strains that are genetically modified for specific purposes. The challenge is that your high value strains often result in continued maintenance even though you may not need them for long periods. Besides, such maintenance may produce excess animals and can be expensive, too.
 
You can avoid these consequences through Envigo’s cryopreservation service and:
 
  • Eliminate maintenance of colonies to sustain available research models
  • Reallocate production space and associated resources to other areas of research priority
  • Preserve the genetic integrity of research models for future colony development
  • Provide insurance against physical, genetic, and pathologic disasters
  • Harvest early stage embryos
  • Preserve a specific number of embryos dependent upon your requirements
  • Store embryos in two geographically separate locations (or we can return all the material to you for preservation)
Services offered:
 
Service Requirements Project outcome
Embryo cryopreservation  using wild type females (Envigo wild type or provided by customer)
  • 5 donor males (6-27 wk)
  • 35 wild-type donor females (3-4 wk)
  • Genotype of donors
Cryopreservation of approximately 300 embryos produced from your male animals and either Envigo wild type females or wild type females you provide. Includes in vitro culture quality control of cryopreserved embryos and dual site storage for first year.
Embryo cryopreservation using customer genetic line (homozygous/heterozygous) females
  • 5 donor males (6-27 wk)
  • 10 donor females (3-4 wk) or
  • Genotype of donors
Cryopreservation of approximately 150 embryos produced from your male and female animals. Includes in vitro culture quality control of cryopreserved embryos and dual site storage for the first year.
Mouse Sperm cryopreservation
  • 2-4 donor males (6-27 wk)
Cryopreservation of mouse sperm. Approximately 22 straws of mouse sperm are provided. Included in vitro fertilization (2 cell embryos production) quality control of cryopreserved sperm and dual site storage for the first year.
Live birth quality control (embryo cryopreservation)
  • Straw of embryos (minimum 40 embryos) thawed
Quality control to generate live offspring to confirm embryo viability.
Live birth quality control (sperm cryopreservation)
  • 2-3 straws of cryopreserved sperm
  • Embryos generated from IVF
Quality control to generate live offspring to confirm sperm viability.

 
Please download our full brochure on contract breeding services for additional information.

For pricing information concerning these services, please fill out our Enbryo Questionnaire or contact us at contractbreeding.eu@envigo.com.