Tyrosine Hydroxylase TH-Cre knockin rat


Characteristics / Husbandry

  • Background strain: Sprague Dawley
  • No observed ectopic expression of cre
  • Targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC

Availability: Live colony
Zygosity genotype: Homozygous

This model expresses cre-recombinase under the control of the endogenous tyrosine hydroxylase promoter enabling specific expression in dopaminergic neurons. This model possesses a targeted insertion of (IRES)-cre immediately after the translational stop in the open reading frame of Th. The TH-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.

We have observed germline excision of floxed alleles in the offspring of female rats with both a floxed allele and at least one Cre allele. We recommend breedings between female rats with homozygous floxed alleles and male rats with both Cre and floxed alleles to obtain offspring that are Th-Cre postive and homozygous for a floxed locus.

Expression/knockout of floxed genes
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The Tyrosine Hydroxylase TH-Cre KI rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo.