5Ht3a-Cre knockin rat


Characteristics / Husbandry

  • Background strain: Long Evans Hooded
  • Cre recombinase driven by endogenous 5Ht3a promoter
  • No observed ectopic expression of cre
  • Targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC

Availability: Live colony
Zygosity genotype: Homozygous

This model expresses cre-recombinase under the control of the endogenous 5’- hydroxytryptamine receptor 3A (5Ht3a) promoter enabling specific expression in 5Ht3a positive serotonergic neurons. This model possesses a targeted insertion of (T2A)-cre immediately before the translational stop in the open reading frame of the 5Ht3a gene. The 5Ht3a-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.

Research Use & Related Publications
Expression/knockout of floxed genes
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The 5Ht3a-Cre KI rat model was originally created at SAGE Labs, Inc. in St. Louis, MO and distributed out of the Boyertown, PA facility. The line continues to be maintained through the original SAGE Labs animal inventory acquired by Envigo.

Technical resources

  • Model information sheet — US