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Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1 – 2 days (non-irradiated) or 1 – 2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don't find a suitable option below.

Formula examples with Teklad global 2018 base diet
Doxycycline hyclate¹ USP (mg/kg diet)	Natural color	Alternatives with food coloring mg per kg diet
46	TD.10483	TD.180327 Red TD.120240 Blue
100	TD.04233	TD.130840 Blue
200	TD.00502	TD.180625 Red TD.150431 Blue TD.04104 Green
625	TD.01306 stocked²	TD.08541 Red TD.120769 Blue TD.09628 Green
1000	TD.05298	TD.06294 Red TD.120658 Blue
2000	TD.05512	TD.140011 Red TD.09633 Green
6000	TD.01533

^{1 Doxycycline hyclate is ~87% doxycycline
2 TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation}

Considerations when choosing a base diet

Teklad Global Rodent Diets® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate¹ at 625 mg/kg diet
Base diet	Natural color	Alternatives with food coloring mg per kg diet
2014	TD.00426	TD.07382 Red TD.07573 Yellow
2016	TD.02503	TD.140263 Blue TD.170364 Red
2019	TD.09651	TD.09761 Green
2020	TD.110720	TD.130141 Red

^{1 Doxycycline hyclate is ~87% doxycycline}

Advantages of diet delivery of doxycycline

Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
Diet is typically changed once per week. Water may need to be changed every few days
Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

Key planning information

Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
Store diet refrigerated and plan to use within six months
Typical lead time is two weeks (four weeks if irradiated)

TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Irradiation (20 - 50 kGy) is optional, and must be requested at time of order

Safe handling of doxycycline diets

Doxycycline is a tetracycline class antibiotic.
Usual dose for therapeutic effects in humans is 100-200 mg/day. Doxycycline diets for rodents typically contain ~2 mg/pellet.
Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.