Cell line posts

Convection-enhanced delivery of an anti-miR is well-tolerated, preserves anti-miR stability and causes efficient target de-repression: a proof of concept (Halle, 2016)

Using a gliobastoma orthotopic xenograft model (athymic nude rat from Envigo), investigators sought to evaluate the efficiency, and safety of miR inhibition using convection-enhanced delivery (CED) of an anti-miR. In vivo, the authors targeted a well-validated mIR (let-7a) via administration of anti-let-7a by CED into orthotopic T87-derived tumors. After one month of infusion, tumors were removed and tumor mRNA levels of the target-gene High-mobility group AT-hook 2 (HMGA2) were determined. The authors found that levels of HMGA2 in the tumor were significantly de-repressed in the anti-miR treated animals, and anti-miR integrity was preserved in the pumps and no animals showed signs of severe adverse effects attributable to the anti-miR treatment. Thus, the authors conclude that these initial proof-of-concept data should be advanced further by using CED for delivery of key oncogenic miRs.

Read the full reference document at www.ncbi.nlm.nih.gov/pubmed/26428358
Further related studies can be found in our cell line database

Proton MR Spectroscopy and Diffusion MR Imaging Monitoring to Predict Tumor Response to Interstitial Photodynamic Therapy for Glioblastoma (Toussaint 2017)

In a recent study, investigators used male athymic nude rats (Hsd:RH-Foxn1rnu) (athymic nude rat from Envigo) with intracranial glioblastoma xenografts to assess tumor response to interstitial photodynamic therapy (iPDT), a site-specific tumor treatment technique that uses laser fibers inserted directly into the depth of the tumor. Rats were injected with AGuIX® nanoparticles (which encapsulate the photodynamic therapy (PDT) and imaging agents) prior to iPDT treatment. Tumor response to iPDT was monitored using non-invasive imaging techniques. Specifically, Magnetic Resonance Imaging (MRI) and Magnetic Resonance Spectroscopy (MRS) were used to follow-up on post-treatment effects over time. Overall, the authors found that conventional MRI, T2 star (T2*), Diffusion Weighted Imaging (DWI), and MRS were all able to extract relevant profiles on tissue cytoarchitectural alterations, local vascular disruption, and metabolic information on brain tumor biology. Together, these markers helped achieve earlier assessment of tumor response. Notably, after only one day post-iPDT, DWI and MRS successfully identified several promising markers, such as Apparent Diffusion Coefficient (ADC) values, lipids, choline and myo-inositol levels, which permitted the researchers to distinguish iPDT responders from non-responders.

Read the full reference document at www.ncbi.nlm.nih.gov/pubmed/28255341
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