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Genetic monitoring reports

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Genetic monitoring is an essential part of inbred colony quality control. Several methods of genetic monitoring are available, including biochemical markers, phenotypic analysis, and, more recently, microsatellite DNA and single nucleotide polymorphism (SNP) analysis. While each method has its advantages, Envigo uses SNP analysis because of the combination of speed, efficiency and sensitivity at detecting genetic contamination of inbred lines.
 
It should be noted that currently available methods of monitoring are relatively insensitive at detecting genetic drift because they sample only a small portion of the genome. To rule out genetic drift, the full genome would have to be sequenced for all animals. All vendor programs for genetic monitoring currently are designed to detect genetic contamination, not genetic drift.

Preventing genetic contamination due to accidental mismating is the first priority for a genetic quality control program. Procedures such as room coat color separation, pest control, and exclusion methods are employed to minimize this risk. Animal care technicians are trained to recognize and report phenotypic deviations. Envigo regularly monitors growth data and production indices that are specific to each model (i.e., detailed production records, pedigrees, and cage cards) to ensure the consistency of our models.

Envigo’s global routine genetic monitoring program consists of collecting tissue samples quarterly from all new pedigreed Foundation Colony breeding cages in non-isolator-bred colonies. Tests are conducted on all pedigreed Foundation Colony breeding cages in newly-populated isolator-bred colonies, and then five (5) new foundation colony breeder cages annually thereafter.
 
Tissue samples are sent to the Envigo laboratory located at the Bionomics Research and Technology Center (BRTC) in Piscataway, New Jersey. The Envigo laboratory employs a customized panel of single nucleotide polymorphisms (SNPs) to evaluate strain differences in both rats and mice. Our routine rat and mouse SNP panels consist of 48 markers. Analysis is performed by utilizing allelic discrimination technology in a fully automated fashion. Our laboratory includes high throughput liquid handling robots that can process up to 500,000 SNPs daily.

Envigo performs routine global genetic monitoring on select outbred stocks, including Hsd:Sprague Dawley® SD®, Hsd:Athymic nude Foxn1rnu, Hsd:LE, RccHan®:WIST, Hsd:ICR(CD-1®), and Hsd:Athymic nude Foxn1nu. These stocks are tested at all locations to compare allelic frequencies both within and between colonies. Forty-eight (48) tissue samples per colony are tested using a panel of 96 SNP markers.